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Saliva RNA Extraction Kit-Alternative Non-invasive Sample Type for COVID-19

 

Saliva RNA Extraction Kit-Alternative Non-invasive Sample Type for New Coronavirus


[Previous situation review]:

 

[1] "One-stop" service for collection and storage of saliva DNA/RNA/exosomes, etc., click to learn

 

[2] Saliva sample processing series 2: Saliva DNA/exosomes extraction kit

 

[3] Norgen Biotek assists in the research of the new coronavirus COVID-19


In mid-April 2020, Rutgers University announced that the U.S. Food and Drug Administration (FDA) has approved the first use of saliva as SARS-CoV-2 (new crown virus) under its emergency use authorization (EUA).

New detection methods for the detection of biological materials.

 

In mid-August 2020, the U.S. FDA announced that it had granted an emergency use authorization (EUA) for the SalivaDirect new crown diagnostic test developed by the Yale University School of Public Health. 

In a press release issued by the FDA, FDA Commissioner Stephen M. Hahn said, This test has made breakthrough progress in improving test efficiency and avoiding reagent shortages.

 

Emergency use authorization for COVID-19 diagnostic test

It is because of the fast speed and low price that this test has been funded by the NBA, and NBA players have tried it out. 

The NBA has reopened and is about to enter the playoffs. Fast, easy, and low-cost nucleic acid monitoring of NBA players and related staff is the key to ensuring the smooth progress of the NBA.

 

The concept of using saliva samples to detect the new coronavirus is not new. The FDA has previously issued EUAs for four molecular diagnostic tests based on saliva samples. Speaking of saliva, it is actually a good test material.

 

Also Refer: Vaccine Development Encyclopedia


When the new coronavirus first broke out, it was reported that the disease was far more infectious than SARS. News reports describe that when a patient gets on the bus, almost one-third will be infected. 

It spreads super fast, and it's distracting. Experts analyze that the viral pathogen may be present in a relatively superficial part, such as breathing and speaking can spread. Then, saliva in the mouth is the most likely. Spreading through droplets is the easiest way. 


Droplet spread of Coronavirus

SARS-CoV-2 virus is the main pathogen of COVID-19, a serious and potentially fatal virus. Nasopharyngeal swab collection, followed by viral RNA extraction and RT-qPCR is the current gold standard workflow for detecting SARS-CoV-2 infection. 

As an invasive sample collection method, this sample collection strategy requires professionally trained personnel on site who are at risk of infection. In the past few months, there has been a shortage of cotton swabs around the world, which provides new challenges for the census.

 

Norgen Biotek uses saliva as a viable alternative non-invasive sample type to optimize the complete workflow to detect SARS-CoV-2 infection. Saliva RNA collection and preservation device, this is the first step of the workflow. No trained personnel is required during the sample collection process. 

The most important thing is that it makes the sample non-infectious and can be safely transported and processed to the greatest extent This reduces the risk to frontline workers. 

Norgen's optimized and validated saliva-based workflow provides a non-invasive, safe and reliable COVID-19 detection method that has been proven to have the same sensitivity and specificity as the current gold standard workflow. 

It makes the samples non-infectious and can be safely transported and processed, thereby minimizing the risk to frontline workers.


 

Three-step method for saliva collection: spit-twist-test

The introduction given here is of the related products of saliva DNA/RNA/exosomes collection and preservation, so how to extract and purify the RNA in saliva after collecting saliva samples to facilitate downstream scientific research? 

As a professional solution provider in the field of life sciences, Amage Technology recommends you a series of products and services for the treatment of saliva samples under the Norgen Biotek brand as the exclusive distributor in Asia.

 

Norgen Biotek saliva sample

Product name and description Item No. Sample size Specification

Saliva/Swab RNA Purification Kit 69 100 250 μL 50 times

Saliva/Swab RNA Purification Kit (CE/IVD Marked) Dx69100 250 μL 50 times

Saliva/Swab RNA Purification 96-Well Kit 69300 250 µL 2 plates

Saliva/Swab RNA Purification 96-Well Kit Dx (CE/IVD Marked) Dx69300 250 µL 2 plates


 

Infographics on Saliva RNA extraction kit

 

1. Features and advantages of saliva/swab RNA purification kit (Cat. No. 69100):

Isolate high-quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples.

Norgen's patented resin separation technology, simple and fast operation;

Isolate total RNA, from large rRNA to microRNA (miRNA).

No need for phenol or chloroform extraction.

Extremely sensitive and linear, can reduce the amount of cell/virus replicons, without carrier RNA.

The purified RNA is suitable for various downstream applications, including small RNA sequencing.

 

 

[Saliva/swab RNA purification kit, manufacturer test results]:

 

 

Infographics o SARS-CoV-2 E gene and RdRP amplification
SARS-CoV-2 E gene and RdRP amplification

SARS-CoV-2 E gene and RdRP amplification

 

Average RNA yield

Average RNA yield of samples isolated with Norgen's saliva/swab RNA purification kit

 

2. Saliva/swab RNA purification 96-well plate kit (article number: 69300) features and advantages:

Isolate high-quality total RNA, including viral RNA, from fresh and preserved saliva and swab samples.

High-throughput 96-well plate format for rapid purification.

Isolate total RNA, from large rRNA to microRNA (miRNA).

Very consistent RNA separation between wells.

No need for phenol or chloroform extraction.

Combine and elute all RNA, regardless of size or GC content, without deviation.

Extremely sensitive and linear, can reduce the amount of cell/virus replicons, without carrier RNA.

The purified RNA is suitable for various downstream applications, including small RNA sequencing.

 

[Saliva/swab RNA purification 96-well plate kit, manufacturer test results]:

 

 

RNase P gene amplification of saliva RNA

Amplification of RNase P gene from fresh and preserved swabs and saliva RNA isolated by this kit

 

Infographics on Human miR-21 amplification
Human miR-21 amplification


Amplification of human miR-21 in fresh and preserved swabs and saliva RNA isolated by this kit


As a biological high-tech enterprise with high-end technical strength, advanced management level and perfect market sales system, it is headquartered in Wuhan Optics Valley High-tech Development Zone and serves the whole country. 

Amage Technology is a professional high-tech company integrating imported reagents, laboratory consumables sales, technical services and contract development. 

It provides users with professional cutting-edge information, complete products, integrated solutions, and high-quality logistics services. 



Author's Bio


Name: Gwynneth May

Educational Qualification: MBBS, MD (Medicine) Gold Medalist

Profession: Doctor

Experience: 16 Years of Work Experience as a Medical Practitioner

About Me


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