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Results of Research on SARS-CoV-2 Targeted Detection Kit

 

Research on SARS-CoV-2 Targeted Detection Kit

The new crown is a common problem and challenge faced by scientists around the world, but in order to overcome it, our scientists have conducted a lot of research on it. As the understanding of COVID-19 has become deeper and deeper, scientists have also developed and produced various Kind of COVID-19 testing products. At present, researchers are turning to the area of ​​concern that SARS-CoV-2 related enzymes contribute to the pathogenicity and spread of COVID-19. Antibody inhibitors derived from these enzymes may play a role in helping to develop treatments.

 

EpiGentek further classifies and organizes the kits for these research directions. At the same time, some new products are gradually attracting people's attention as a way to better understand how to fight viruses. There are currently three main ways:

 

1. SARS-CoV-2 proprotein convertase (PC) activity and inhibition analysis kit

The SARS-CoV-2 spike glycoprotein has a furin/PC cleavage site on the boundary of the S1/S2 subunits, which can be cleaved by furin secreted by host cells and bacteria and/or furin-like PCs. 

Unlike SARS-CoV, SARS-CoV-2 entering cells is pre-activated by furin and/or furin-like pc, reducing its dependence on target cell protease entry. 

It has been proven that the lysis and activation of S protein is essential for the SARS-CoV-2 spike-mediated virus-ACE2 binding, cell-cell fusion, and virus entry into human lung cells.


 

SARS-CoV-2 preprotein convertase (PC) activity and inhibition analysis kit

Significance: Furin and/or PC-based SARS-CoV-2 S1/S2 lysis increases the entry and replication of SARS-CoV-2 into cells and eventually develops higher COVID-19 pathogenicity. Therefore, the detection of SARS-CoV-2 targeting Furin/PC lytic activity will help determine why the sensitivity and pathogenicity of COVID-19 are different in different individuals. 

The screening of inhibitors will help develop effective COVID-19 treatment drugs by blocking the SARS-CoV-2 lysis at the S1/S2 boundary.

 

EpiGentek provides two new kits to observe the inhibitory effect of pro-proteolysis activation from different angles.

 

Kit Type

Kit Details

Furin protease/PC inhibitor screening

SARS-CoV-2-targeted protease cleavage activity and inhibition detected using theCoviDrop™ SARS-CoV-2 Targeted Proprotein Convertase Inhibitor Screening Fast Kit. Concentration (ng/well): Furin: 10 ng. Plasmin: 50 ng; Trypsin: 50 ng.

 

Protease Inhibitor: 500 ng.

Fast colorimetric screening: intuitive 2-hour program

Advantages of CoviDrop SARS-CoV-2 targeted proprotein convertase inhibitor rapid screening kit:

1. Used for high-throughput screening of compounds that inhibit the cleavage of SARS-CoV-2 original protein convertase 2. Sensitivity: from as low as

Activity is detected in 2 ng of PC or promoted serine protease (such as HAT)

3. Specificity: The substrate contains the complete SARS-CoV-2 S1/S2 cleavage sequence, which is proved to be the same as the real spike protein with tertiary structure in the PC cleavage test. This means that the assay has unique specificity for the detection of SARS-CoV-2-S1/S2 cleavage and is suitable for measuring the inhibitory effect of various inhibitors on the cleavage of PC targeting SARS-CoV-2

4. Flexible: microplate format with wells for manual or high-throughput analysis

Use biological samples for Furin protease/PC lysis activity and inhibition assay

Illustrated dose-response of Positive Control Enzyme cleavage activity assayed with the CoviDrop™ SARS-CoV-2 Targeted Proprotein Convertase Activity/Inhibition Assay Kit.

Fast measurement process: only 50 minutes to complete the entire process

 

 

2. SARS-CoV-2 total IgG/IgM and neutralizing antibody measurement kit

SARS-CoV-2 infection usually induces an antibody response. The antibody can bind to SARS-CoV-2 spikes, and only a small part has a neutralizing function. Only neutralizing antibodies can block the effect of SARS-CoV-2 spikes and inhibit the SARS-CoV-2 virus from infecting new cells.

 

SARS-CoV-2 total IgG/IgM and neutralizing antibody measurement kit

Significance: Measuring the total SARS-CoV-2 specific IgG/IgM helps determine whether an individual is infected with SARS-Cov-2. 

Infographics on Results of Research on SARS-CoV-2 Targeted Detection Kit


However, it is more important to detect SARS-CoV-2 neutralizing antibodies in a quantitative and high-throughput format:

 

1.) It can help determine whether symptomatic infected individuals have acquired protective immunity from COVID-19 and how long the neutralizing antibody persists after infection

 

2.) It helps to accurately evaluate therapeutic antibodies against SARS-CoV-

 

3.) This will greatly benefit the development of effective vaccines.


EpiGentek provides two different types of kits for the quantitative measurement of SARS-CoV-2 antibodies in human samples. 

The first is the SeroFlash SARS-CoV-2 IgG / IgM ELISA rapid kit, which can quickly measure SARS-CoV-2 IgG and IgM antibodies from plasma or serum sample input. 

The other is the SeroFlash SARS-CoV-2 neutralizing antibody rapid determination kit, which can quickly quantify the amount of specific SARS-CoV-2 neutralizing antibodies from samples including serum, plasma and various body fluids.

 

Kit Type

Kit Details

IgG / IgM antibody quantification

 

Cat#: D-1002

SeroFlash SARS-CoV-2 IgG / IgM ELISA

High sensitive detection of SARS-CoV-2 IgG.

 

Humanized anti-SARS-CoV-2 IgG was spike to human serum at different concentration and then detected with SeroFlash™ SARS-CoV-2 IgG ELISA Fast Kit.

SeroFlash SARS-CoV-2 IgG / IgM ELISA Rapid Kit

For high-throughput qualitative screening of the presence of SARS-CoV-2 antibodies in human serum/plasma.

 

1. Fast: the whole process can be completed in less than 45 minutes

 

2. High sensitivity: the limit of detection (LoD) can be as low as 0.002 µg / ml SARS-CoV-2 IgG antibody

 

3. Specific: specific to SARS-CoV-2 IgG and IgM, no cross-reactivity with many other viruses.

 

4. Flexible: microplate format with wells for manual or high-throughput analysis

Neutralizing antibody quantification

 

Cat #: D-1008

SeroFlash SARS-CoV-2 IgG / IgM ELISA

Inhibition of SARS-CoV-2-ACE2 binding by positive neutralizing antibody detected with the SeroFlash™ SARS-CoV-2 Neutralizing Antibody Assay Fast Kit.

SeroFlash SARS-CoV-2 Neutralizing Antibody Determination Rapid Kit

Detect and quantitatively measure SARS-CoV-2 neutralizing antibodies from various human samples

 

1. Convenient and fast: The easy-to-follow process can be completed in 50 minutes.

 

2. Sensitive and specific: the neutralizing antibody level can detect as low as 1 ng, and is resistant to SARS-CoV-2

 

3. With specificity, it is suitable for identifying whether there are neutralizing antibodies in infected individuals and distinguishing SARS-CoV-2 neutralizing antibodies from other antibodies.

 

4. Quantification: including determination standards, which can quantify the titer or concentration of neutralizing antibody for EC50 calculation.

 

5. Flexible: microplate format with wells for manual or high-throughput analysis

 

In addition to Epigentek, these products include Abnova, lifesensor, etc.

 


Brand

Products

Item Number

Abnova

COVID-19 Human IgM IgG Rapid Test

Cat#: DC0301

COVID-19 Human IgM IgG Assay Kit

Cat #: KA5826

Lifesensor

COVID-19 Human IgM IgG Assay Kit DELUXE

Cat#:DE0002

COVID-19 IgG ELISA Detection Kit

Cat#: CV3002

COVID-19 IgG ELISA Research Kit

Cat#: CV5002

 

 

3. SARS-CoV-2 Spike-ACE2 binding activity and inhibition analysis kit

The process of SARS-CoV-2 entering human host cells occurs through the binding of the surface unit S1 of its spike protein to the cell receptor angiotensin converting enzyme 2 (ACE2). 

ACE2 is ubiquitous and is widely expressed at different levels in the lungs, heart, kidneys, blood vessels, brain, intestines and testes. 

ACE2 also mainly binds to cell membranes and is usually less present in the circulation. It has been found that the increased expression of ACE2 in the nasal epithelium and lungs is related to the higher rate of SARS-CoV-2 infection and the severity of COVID-19, respectively. 

A new observation suggests that increased circulating ACE2 concentrations may be related to increased incidence and mortality of COVID-19. 

In addition, SARS-CoV-2 invasion will degrade ACE2 on the cell membrane and may increase the concentration of soluble ACE2 in the circulation.


 

SARS-CoV-2 Spike-ACE2 binding activity and inhibition analysis kit

Significance: SARS-CoV-2-ACE2 binding activity determination will truly reflect the level of functional ACE2 in binding to SARS-CoV-2. 

This assay can not only detect ACE2 binding activity under healthy and diseased conditions, but also measure changes in ACE2 levels under various experimental interventions, including in vivo ACE2 epigenetic regulation, in vivo and in vitro ACE2 activity inhibition (such as: antibodies and Small molecule inhibitors) and environmental impact (e.g.: smoking).

 

It is also due to the complex interaction of SARS-CoV-2-ACE2 under normal (healthy) and abnormal (previous diseases and viral infections), as well as the current COVID-19 pandemic and its possible recovery. 

Inhibitor screening is necessary to help develop effective drugs, vaccines and therapeutic antibodies that target COVID-19.

 

EpiGentek provides two new kits: CoviDrop SARS-CoV-2 Spike-ACE2 Binding Inhibitor Rapid Screening Kit, which can help quickly screen out Spike-ACE2 binding immunological or chemical inhibitors. And CoviDrop SARS-CoV-2 Spike-ACE2 binding activity/inhibition assay kit, used to measure Spike-ACE2 binding activity and inhibition of biological samples. 

Each assay kit provides a fast, easy-to-follow procedure that can be used for manual or high-throughput analysis.

 

Kit Type

Kit Details

Screening of SARS-CoV-2-ACE2 binding inhibitors

Cat #: D-1004

CoviDrop SARS-CoV-2 Spike-ACE2 binding inhibitor rapid screening kit

Illustrated dose-response of SARS-CoV-2-ACE2 binding assayed with the CoviDrop SARS-CoV-2 Spike-ACE2 Binding Inhibitor Screening Fast Kit

CoviDrop SARS-CoV-2 Spike-ACE2 binding inhibitor screening rapid kit

Rapid screening of SARS-CoV-2 Spike-ACE2 inhibitors based on colorimetry.

 

1. Fast: easy to follow 1 hour and 30 minutes procedure

 

2. Sensitive and specific: activity can be detected from as low as 0.1 ng of ACE2 conjugate, and it has specificity for SARS-CoV-2-ACE2 binding

 

3. Quantitative: Including measurement standards, ACE24 that can be combined quantitatively. Flexible: well-equipped microplate format for manual or high-throughput analysis

Detection of SARS-CoV-2-ACE2 binding activity and inhibition of biological samples

Cat #: D-1005

Illustrated dose-response of SARS-CoV-2-ACE2 binding assayed with the CoviDrop SARS-CoV-2 Spike-ACE2 Binding Activity/Inhibition  Assay Kit.

CoviDrop SARS-CoV-2 Spike-ACE2 binding activity/inhibition assay kit

Quantitative measurement of SARS-CoV-2-ACE2 binding activity using various human samples

 

1. Fast: easy to follow 1 hour 45 minutes program

 

2. Sensitive and specific: from as low as 0.1 ng of ACE

2 Activity is detected in the conjugate and is only specific for ACE2

 

3. Convenience: You can use biological samples, including plasma, serum, swab samples, other body fluids and purified proteins, to detect the binding activity/inhibition of SARS-CoV-2-ACE2 in vivo and in vitro.

 

4. Flexible: microplate format with wells for manual or high-throughput analysis

 

As a biological high-tech enterprise with high-end technical strength, advanced management level and perfect market sales system, it is headquartered in Wuhan Optics Valley High-tech Development Zone and serves the whole country. 

Amage Technology is a professional high-tech company integrating imported reagents, laboratory consumables sales, technical services and contract development. 

It provides users with professional cutting-edge information, complete products, integrated solutions, and high-quality logistics services. 

In order to better serve customers, the company has established an experienced R&D team-Immage Biotechnology Center, entered the R&D and production stage, and recommended better products to colleagues in the biological field.

 

Amy Jet Technology maintains a close cooperative relationship with excellent biological reagent suppliers, and has become the general agent or first-level agent in China for many internationally renowned brands, including:

AmyJet, AAT Bioquest, Abnova, Agrisera, Biovision , Biosensis, Cayman Chemical, Caisson Labs, Cytoskeleton, CytoNiChe, Epigentek, Equitech-Bio, Hycult Biotech, Jackson, LifeSensors, LigaTrap, ProSpec, Norgen Biotek, Mabtech, Matreya, iRegene, StressMarq, ImmunoReagent, Southern BioReagent, Ori Solis BioDyne, etc., can provide users with professional cutting-edge information, complete products and logistics services in a short time.

 

  

Author's Bio


Name: Gwynneth May

Educational Qualification: MBBS, MD (Medicine) Gold Medalist

Profession: Doctor

Experience: 16 Years of Work Experience as a Medical Practitioner

About Me


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